Dot-blot hybridization analysis of subtracted fragments membranes were screened by hybridization with genomic DNA from the tester (panel A) and the driver. RNA slot-blot hybridization of two cotton cultivars, Giza 45 and Giza 84, performed by use of their respective related probes G45 an d G84 probes. The dot blot hybridization assay using the nonradioactive probe led to similar results to indirect immunofluorescence (IFI) when tested against a panel of. Dot-blot hybridization method definition, Principle, steps, advantages A simplified 3 minute videoThe video explainsDot-blot hybridization. Fig. Detection of plant RNA viruses by nonisotopic dot-blot hybridization. Additional Online Courses. Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. . Polymerase chain reaction (PCR) provides a powerful technique for identifying viruses and studying the homology between viral nucleic acids. Slot Blot Hybridization · AluQuant · Quantitative PCR · Data Analysis for Quantitative PCR · Summary of Advantages and Disadvantages.